The western blotting procedures were fully described previously.
14 (link) The following primary antibodies were used: anti‐4‐HNE (Abcam, AB46545, 1:4000), anti‐Bax (Santa Cruz, Sc‐7480, 1:100), anti‐Bcl‐2 (Santa Cruz, Sc‐7382, 1:200), anti‐catalase (GeneTex, GTX110704, 1:1000), anti‐citrate synthase (Santa Cruz, Sc‐390693, 1:200), anti‐p‐DRP1 (Ser616, Cell Signaling, #34555, 1:500), anti‐DRP1 (Cell Signaling, #5391S, 1:500), anti‐Fis1 (Santa Cruz, Sc‐376447, 1:200), anti GPx1 (Thermo Fisher Scientific, PA5‐26323, 1:1000), anti‐MFN2 (Santa Cruz, Sc‐100560, 1:200), anti‐Mul1 (Abcam, AB209263, 1:1000), anti‐OPA1 (Thermo Fisher Scientific, MA5‐16149, 1:1000), anti‐Parkin (Abcam, AB77924, 1:2000), anti‐PINK1 (Thermo Fisher Scientific, PA1‐16604, 1:500), anti‐PGC‐1α1 (Millipore, AB3242, 1:1000), anti‐SOD2 (Genetex, GTX116093, 1:1000) and anti‐VDAC (Santa Cruz, Sc‐390996, 1:200). Then, anti‐rabbit (Cell Signaling, #7074S, 1:4000) or anti‐mouse (Cell Signaling, #7076S, 1:4000) secondary antibodies were used. The blots were revealed using a Pierce ECL kit (Thermo Fisher Scientific) or SupraSignal Femto kit (Thermo Fisher Scientific), and proteins were visualized by enhanced chemiluminescence (iBright 1500 Imaging System, Invitrogen) and quantified with ImageJ Software (version 1.8.0). Ponceau coloration was used as the loading control.
14 (link) The following primary antibodies were used: anti‐4‐HNE (Abcam, AB46545, 1:4000), anti‐Bax (Santa Cruz, Sc‐7480, 1:100), anti‐Bcl‐2 (Santa Cruz, Sc‐7382, 1:200), anti‐catalase (GeneTex, GTX110704, 1:1000), anti‐citrate synthase (Santa Cruz, Sc‐390693, 1:200), anti‐p‐DRP1 (Ser616, Cell Signaling, #34555, 1:500), anti‐DRP1 (Cell Signaling, #5391S, 1:500), anti‐Fis1 (Santa Cruz, Sc‐376447, 1:200), anti GPx1 (Thermo Fisher Scientific, PA5‐26323, 1:1000), anti‐MFN2 (Santa Cruz, Sc‐100560, 1:200), anti‐Mul1 (Abcam, AB209263, 1:1000), anti‐OPA1 (Thermo Fisher Scientific, MA5‐16149, 1:1000), anti‐Parkin (Abcam, AB77924, 1:2000), anti‐PINK1 (Thermo Fisher Scientific, PA1‐16604, 1:500), anti‐PGC‐1α1 (Millipore, AB3242, 1:1000), anti‐SOD2 (Genetex, GTX116093, 1:1000) and anti‐VDAC (Santa Cruz, Sc‐390996, 1:200). Then, anti‐rabbit (Cell Signaling, #7074S, 1:4000) or anti‐mouse (Cell Signaling, #7076S, 1:4000) secondary antibodies were used. The blots were revealed using a Pierce ECL kit (Thermo Fisher Scientific) or SupraSignal Femto kit (Thermo Fisher Scientific), and proteins were visualized by enhanced chemiluminescence (iBright 1500 Imaging System, Invitrogen) and quantified with ImageJ Software (version 1.8.0). Ponceau coloration was used as the loading control.
