Conditional Deletion of Pfkfb3 in Myeloid Cells

Mice were used in accordance with the protocol approved by the Institutional Animal Care and Use Committee of Augusta University. The floxed Pfkfb3 (Pfkfb3^flox/flox) mice were generated by Xenogen Biosciences Corporation (Cranbury, NJ, USA) (14 (link)). Myeloid-specific Pfkfb3 knockout was achieved by cross-breeding Pfkfb3^flox/flox mice with Lysm-Cre mice (stock no. 004781, The Jackson Laboratory, Bar Harbor, ME, USA) to generate Pfkfb3^flox/flox; Lysm-Cre (Pfkfb3^ΔMϕ) mice. All mice were on a C57BL/6J background.

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Xu J., Wang L., Yang Q., Ma Q., Zhou Y., Cai Y., Mao X., Da Q., Lu T., Su Y., Bagi Z., Lucas R., Liu Z., Hong M., Ouyang K, & Huo Y. (2021). Deficiency of Myeloid Pfkfb3 Protects Mice From Lung Edema and Cardiac Dysfunction in LPS-Induced Endotoxemia. Frontiers in Cardiovascular Medicine, 8, 745810.

Publication 2021

Lysm-Cre mice Lysm-Cre

Institutional animal care and use committee Mice

Corresponding Organization : Augusta University

Other organizations : Peking University, Peking University Shenzhen Hospital, Emory University, Jinan University

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Variable analysis

independent variables

Pfkfb3 knockout in myeloid cells

dependent variables

Not explicitly mentioned

control variables

Mouse strain (C57BL/6J background)
Pfkfb3 flox/flox mice
Lysm-Cre mice

controls

Pfkfb3 flox/flox mice (positive control)
Lysm-Cre mice (negative control)

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