Bisulfite sequencing was conducted as described [50 (link)]. Briefly, extracted DNA samples were bisulfite converted using the Epitect Bisulfite Kit Protocol (Qiagen, Düsseldorf, Germany). The sequencing service was provided by Tech Dragon Ltd., Hong Kong. The PCR primers were listed as follows: NKX6−2 forward: GGTTTGGAAAAGTTATTTG; NKX6−2 reverse: AAACTACACCTCAATAAAACC.
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