mRNA from was isolated from epidermis of 3–6 animals per group and pooled. The Affymetrix GeneChip Mouse Gene 2.0 ST Array (Thermo Fisher Scientific, Waltham, MA, USA) was used according to the manufacturer’s instructions. A Custom CDF V. 22 with ENTREZ-based gene definitions was used to annotate the arrays [32 (link)]. Quantile normalization and robust multiarray analysis background correction were used for normalization of fluorescence intensity. An analysis of variance was performed to identify differentially expressed genes using the commercial software package SAS JMP Genomics, V. 7 (SAS Institute, Cary, NC, USA). The level of significance was a 0.05 false positive rate with false discovery rate correction (GEO accession number: GSE161649).
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