Protein Expression Analysis via Immunoblotting

Immunofluorescence and immunoblotting, on SDS-PAGE gels were performed as reported previously (Hoare et al., 2016 (link)). The following antibodies were used in this study: anti-COX2 (Cell Signaling, 12282, 1:1000); anti-HRAS (Calbiochem, OP-23, 1:500); anti-IL1a (R&D systems, MAB200, 1:100); anti-IL6 (R&D systems, MAB2061, 1:250); anti-IL8 (R&D systems, MAB208, 1:500); anti-β-Actin (Sigma, A5441, 1:5000); anti-C/EBPβ (Santa-Cruz, sc-150, 1:500); anti-IkBα (Cell Signaling, 4814, 1:1000); anti-PGE₂ (Abcam, ab2318, 1:100); anti-CyclinA2 (Sigma, C4710, 1:500).

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Gonçalves S., Yin K., Ito Y., Chan A., Olan I., Gough S., Cassidy L., Serrao E., Smith S., Young A., Narita M, & Hoare M. (2021). COX2 regulates senescence secretome composition and senescence surveillance through PGE2. Cell Reports, 34(11), 108860.

Publication 2021

anti-COX2 MAB200 anti-IL6 MAB2061 anti-IL8 anti-β-Actin anti-C/EBPβ anti-IkBα anti-PGE2 anti-CyclinA2

Actin Antibodies Cox2 Gels Hras Il1a Immunofluorescence Pge2 Sds page

Corresponding Organization : University of Cambridge

Other organizations : Tokyo Institute of Technology

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of COX2, HRAS, IL1a, IL6, IL8, β-Actin, C/EBPβ, IkBα, PGE2, and CyclinA2

control variables

None explicitly mentioned

controls

Positive controls: None mentioned
Negative controls: None mentioned

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