Serum Biomarkers and Kidney Function

After overnight fasting for 8–12 h, blood samples of approximately 5 mL were obtained from participants and immediately centrifuged at 3000× g for 10 min. Serum levels of blood urea nitrogen (BUN), creatinine, triglycerides, total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), fasting glucose, total calcium, phosphorus and C-reactive protein (CRP) were measured by an autoanalyzer (Siemens Advia 1800; Siemens Healthcare GmbH, Henkestr, Germany) [17 (link),18 (link)]. Serum ANGPTL3 (R&D Systems, Inc., Minneapolis, MN, USA) and intact parathyroid hormone (iPTH) levels (Abcam, Cambridge, MA, USA) concentrations were measured using commercial enzyme-linked immunosorbent assay [17 (link),18 (link)]. The estimated glomerular filtration rate (eGFR) was estimated from the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation.

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Hsiao C.H., Chen Y.C., Wang J.H, & Hsu B.G. (2021). Serum Angiopoietin-like Protein 3 Level Is Associated with Peripheral Arterial Stiffness in Patients with Coronary Artery Disease. Medicina, 57(10), 1011.

Publication 2021

Siemens Advia 1800 ANGPTL3

Blood Blood urea nitrogen C reactive protein Calcium Cholesterol Creatinine Enzyme linked immunosorbent assay Glomerular filtration rate Glucose High density lipoprotein cholesterol Low density lipoprotein cholesterol Parathyroid hormone Phosphorus Serum Triglycerides

Corresponding Organization : Tzu Chi University

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Variable analysis

independent variables

Overnight fasting for 8–12 h

dependent variables

Serum levels of blood urea nitrogen (BUN)
Serum levels of creatinine
Serum levels of triglycerides
Serum levels of total cholesterol
Serum levels of high-density lipoprotein cholesterol (HDL-C)
Serum levels of low-density lipoprotein cholesterol (LDL-C)
Serum levels of fasting glucose
Serum levels of total calcium
Serum levels of phosphorus
Serum levels of C-reactive protein (CRP)
Serum levels of ANGPTL3
Serum levels of intact parathyroid hormone (iPTH)
Estimated glomerular filtration rate (eGFR)

control variables

Centrifugation at 3000× g for 10 min
Autoanalyzer (Siemens Advia 1800; Siemens Healthcare GmbH, Henkestr, Germany) for measuring serum levels
Commercial enzyme-linked immunosorbent assay for measuring serum ANGPTL3 and iPTH levels

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