Tracing EGFP-Positive Neuronal Circuits

C57BL/6J male mice at age P56 were injected with EGFP-expressing AAV using iontophoresis by the stereotaxic method. The brains were scanned using the STP tomography systems. Images were subject to data quality control and all the injection sites were manually annotated. All the image sets were co-registered into the 3D reference space. EGFP-positive signals were segmented from background, and binned at voxel levels for quantitative analyses. The raw connectivity data are served with various navigation tools on the web through the Allen Institute’s data portal. See the full Methods section for detailed descriptions.

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Oh S.W., Harris J.A., Ng L., Winslow B., Cain N., Mihalas S., Wang Q., Lau C., Kuan L., Henry A.M., Mortrud M.T., Ouellette B., Nguyen T.N., Sorensen S.A., Slaughterbeck C.R., Wakeman W., Li Y., Feng D., Ho A., Nicholas E., Hirokawa K.E., Bohn P., Joines K.M., Peng H., Hawrylycz M.J., Phillips J.W., Hohmann J.G., Wohnoutka P., Gerfen C.R., Koch C., Bernard A., Dang C., Jones A.R, & Zeng H. (2014). A mesoscale connectome of the mouse brain. Nature, 508(7495), 207-214.

Publication 2014

Brains C57bl mice Injection sites Iontophoresis Male Stereotaxic method Tomography systems

Corresponding Organization :

Other organizations : Allen Institute for Brain Science, National Institutes of Health

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Variable analysis

independent variables

Injection of EGFP-expressing AAV using iontophoresis by the stereotaxic method

dependent variables

EGFP-positive signals
Connectivity data

control variables

Age of the mice (P56)
Strain of mice (C57BL/6J)
Sex of mice (male)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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