Tissue Preparation and Preservation Protocol

At the time of sacrifice, fresh tissues from the mediastinal lymph nodes, liver, spleen, and kidneys were prepared for culture. The lungs were resected en-bloc and inspected visually for areas of inflammation. One lobe that appeared abnormal was isolated, tied off, and resected for culture. The remaining segments of lung were suspended after cannulating the trachea and then inflated with formalin infused at a height of 30 centimeters. After inflation, the tracheal was tied off and the lungs; as well as sections from the liver, spleen, and kidneys were submerged in 100% formalin for a period of 10 days. All tissues were then sent to the Central Pathology Lab at the UTHSC-SA for processing and staining.

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Peters J., Maselli D.J., Mangat M., Coalson J.J., Hinojosa C., Giavedoni L., Brown-Elliott B.A., Chan E, & Griffith D. (2023). A marmoset model for Mycobacterium avium complex pulmonary disease. PLOS ONE, 18(3), e0260563.

Publication 2023

Formalin Inflammation Kidneys Liver Lungs Lymph nodes Mediastinal Spleen Tissues Tracheal

Corresponding Organization : National Jewish Health

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Variable analysis

independent variables

Resection of one lobe of the lung that appeared abnormal

dependent variables

Culture of tissues from the mediastinal lymph nodes, liver, spleen, and kidneys
Culture of the resected lung lobe
Visual inspection of the lungs for areas of inflammation
Histological processing and staining of the remaining lung segments, liver, spleen, and kidneys

control variables

Inflation of the remaining lung segments with formalin infused at a height of 30 centimeters
Submersion of the lungs, liver, spleen, and kidneys in 100% formalin for 10 days

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