All the experiments were approved by the ethical committee from the university and conform with regulatory standards (LA1230406, project 666N). Mice were bred and maintained under pathogen-free conditions in a certified animal facility in accordance with the European guidelines. Adult (8-week-old) wild-type CD1 mice or Krt8-YFP knock-in mice (18 (link)) were used for sequencing and histology. Krt5-CreERT2 knock-in mice (the Jackson Laboratory, stock no. 029155; RRID:IMSR_JAX:029155) were crossed with R26R-EYFP (the Jackson Laboratory, stock no. 006148; RRID:IMSR_JAX:006148) to generate Krt5-CreERT2:R26R-EYFP (K5:RYFP) mice. For lineage tracing experiments, 10 or 0.25 mg of TAM resuspended in sunflower oil was injected intraperitoneally to 8-week-old K5:RYFP mice, and the expression of the YFP was analyzed in esophagi up to 4 weeks after TAM administration. In all the experiments, littermates of the same sex were randomly assigned to experimental groups.
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Krt5-CreERT2:R26R-EYFP Lineage Tracing
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Corresponding Organization : Institut Belge pour la Sécurité Routière
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Variable analysis
independent variables
- Genotype (wild-type CD1 mice or Krt8-YFP knock-in mice)
- Dose of tamoxifen (10 mg or 0.25 mg)
- Expression of YFP in esophagi
- Age of mice (8 weeks old)
- Sex of mice (littermates of the same sex)
- Housing conditions (pathogen-free, certified animal facility)
- Regulatory standards (LA1230406, project 666N)
- Ethical approval (from the university)
- Krt5-CreERT2:R26R-EYFP (K5:RYFP) mice
- Not specified
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