124I was either provided in-house by the Memorial Sloan Kettering Radiochemistry & Molecular Imaging Probes Core Facility or purchased commercially (IBA Molecular). Stocks of hu3F8-C825 were labeled with radioactive iodine using pre-coated IODOGEN tubes (Pierce) according to standard protocols (15 , 16 (link)) to final specific activities of 121-152 MBq/mg. The tracer immunoreactivities were evaluated using cell-binding assays (17 (link)) and were 78.8 ± 0.1% (n = 3) for GD2(+)-NB IMR-32 and 4.9 ± 0.4% (n = 3) for GD2(-) SK-N-SH. The chelate p-aminobenzyl-DOTA (DOTA-Bn; Macrocyclics) was radiolabeled with 177LuCl3 (specific activity: 170 MBq/nmol; Perkin Elmer) at a ratio of 37 MBq to 1.1 μmol DOTA-Bn using previously described methods (9 (link)), but without HPLC purification (C825 does not recognize metal-free DOTA-Bn).
Protocol detail
Labeling of hu3F8-C825 with Radioactive Iodine and Lutetium-177
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Memorial Sloan Kettering Cancer Center
Top 5 similar protocols
Variable analysis
independent variables
- Radiolabeling of hu3F8-C825 with 124I using pre-coated IODOGEN tubes
- Radiolabeling of
p -aminobenzyl-DOTA (DOTA-Bn) with 177LuCl3
- Specific activities of the radiolabeled hu3F8-C825 (121-152 MBq/mg)
- Immunoreactivities of the radiolabeled hu3F8-C825 (78.8 ± 0.1% for GD2(+)-NB IMR-32 and 4.9 ± 0.4% for GD2(-) SK-N-SH)
- Standard protocols for radiolabeling of hu3F8-C825 and DOTA-Bn
- Absence of HPLC purification for the 177Lu-DOTA-Bn due to the lack of recognition of metal-free DOTA-Bn by C825
- GD2(+)-NB IMR-32 cell line for evaluating the immunoreactivity of radiolabeled hu3F8-C825
- GD2(-) SK-N-SH cell line for evaluating the immunoreactivity of radiolabeled hu3F8-C825
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!