Extraction and quantification of taxanes were conducted by the method previously described [15 (link)].
For extraction and analysis of phenolic acids and flavonoids the samples were homogenized in MeOH containing 1% AcOH and were incubated overnight at room temperature. The supernatant was separated by centrifugation (10,000×g, 15 min, 4°C), dried, and re-dissolved in MeOH before applying for HPLC analysis. Phenolic acids were eluted by a gradient (5%-100%) MeOH: Water (consists of 2% AcOH) with a flow rate of 1 mL min-1, at 287 and 300 nm.
The flavonoids were eluted with a linear gradient (18%-82%) of acetonitrile (MeCN): distilled water (containing 0.5% O-phosphoric acid), with a rate flow of 0.8 mL min-1, and detected at 280 and 350 nm [18 (link)].
For extraction and analysis of phenolic acids and flavonoids the samples were homogenized in MeOH containing 1% AcOH and were incubated overnight at room temperature. The supernatant was separated by centrifugation (10,000×g, 15 min, 4°C), dried, and re-dissolved in MeOH before applying for HPLC analysis. Phenolic acids were eluted by a gradient (5%-100%) MeOH: Water (consists of 2% AcOH) with a flow rate of 1 mL min-1, at 287 and 300 nm.
The flavonoids were eluted with a linear gradient (18%-82%) of acetonitrile (MeCN): distilled water (containing 0.5% O-phosphoric acid), with a rate flow of 0.8 mL min-1, and detected at 280 and 350 nm [18 (link)].
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