Ion Concentration Analysis Protocol

The ion concentrations were analyzed using commercial one-component reagents:

–

Calcium (Alpha Diagnostics, arsenazo III method, reagent composition: TRIS buffer pH 8.5; arsenazo III, 8-hydroxy-quinoline-5-sulfonic acid, inactive stabilizers and detergents 630–670 nm);

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Magnesium (Alpha Diagnostics Magnesium Xylidyl Blue, reagent composition: trioglycolic acid, DMSO, Xylidyl Blue, measurement at 550 nm);

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Phosphorus (Alpha Diagnostics, direct method with phosphomolybdate, reagent composition: sulfuric acid, ammonium molybdate, measurement at 340 nm).

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Skowronek P, & Strachecka A. (2023). Cannabidiol (CBD) Supports the Honeybee Worker Organism by Activating the Antioxidant System. Antioxidants, 12(2), 279.

Publication 2023

Acid Ammonium molybdate Arsenazo iii Calcium Detergents Diagnostics Dmso Magnesium Phosphomolybdate Phosphorus Quinoline Sulfonic acid Sulfuric acid Tris buffer

Corresponding Organization : University of Life Sciences in Lublin

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Calcium concentration
Magnesium concentration
Phosphorus concentration

control variables

Not explicitly mentioned

controls

No positive or negative controls were mentioned in the input protocol.

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