Hematological Parameters in Experimental Fish

During the experimental period, several hematological parameters such as red blood cells (RBCs), white blood cells (WBCs), hemoglobin (Hb), and mean corpuscular hemoglobin (MCH) were measured. The blood was collected through a micropipette cutting caudal peduncle of the experimental fish. To prevent blood clotting hematocrit tube was used to draw the blood [41 (link)]. The collected samples were kept in Eppendorf tubes and stored in a refrigerator (at 4 °C temp.). RBCs and WBCs count were done with the help of Neubauer Hemocytometer under light microscope following the process of Hesser [41 (link)]. Hb concentration was measured using cyan-methemoglobin following the method described by Blaxhall and Daisley [42 (link)]. RBCs, WBCs, and MCH were estimated through the following formula [43 (link)]- $\mathrm{R}\mathrm{B}\mathrm{C}\mathrm{c}\mathrm{o}\mathrm{u}\mathrm{n}\mathrm{t}=\frac{\mathrm{N}\mathrm{u}\mathrm{m}\mathrm{b}\mathrm{e}\mathrm{r}\mathrm{o}\mathrm{f}\mathrm{l}\mathrm{a}\mathrm{r}\mathrm{g}\mathrm{e}\mathrm{s}\mathrm{q}\mathrm{u}\mathrm{a}\mathrm{r}\mathrm{e}\mathrm{c}\mathrm{e}\mathrm{l}\mathrm{l}\left(5\right)\times \mathrm{D}\mathrm{i}\mathrm{l}\mathrm{u}\mathrm{t}\mathrm{i}\mathrm{o}\mathrm{n}\mathrm{f}\mathrm{a}\mathrm{c}\mathrm{t}\mathrm{o}\mathrm{r}\left(200\right)\times \mathrm{C}\mathrm{o}\mathrm{u}\mathrm{n}\mathrm{t}\mathrm{i}\mathrm{n}\mathrm{g}\mathrm{f}\mathrm{a}\mathrm{c}\mathrm{t}\mathrm{o}\mathrm{r}\left(4000\right)}{\mathrm{N}\mathrm{u}\mathrm{m}\mathrm{b}\mathrm{e}\mathrm{r}\mathrm{o}\mathrm{f}\mathrm{s}\mathrm{m}\mathrm{a}\mathrm{l}\mathrm{l}\mathrm{s}\mathrm{q}\mathrm{u}\mathrm{a}\mathrm{r}\mathrm{e}\mathrm{s}\mathrm{c}\mathrm{o}\mathrm{u}\mathrm{n}\mathrm{t}\mathrm{e}\mathrm{d}\left(80\right)}$ $\mathrm{W}\mathrm{B}\mathrm{C}\mathrm{c}\mathrm{o}\mathrm{u}\mathrm{n}\mathrm{t}=\frac{\mathrm{N}\mathrm{u}\mathrm{m}\mathrm{b}\mathrm{e}\mathrm{r}\mathrm{o}\mathrm{f}\mathrm{l}\mathrm{a}\mathrm{r}\mathrm{g}\mathrm{e}\mathrm{s}\mathrm{q}\mathrm{u}\mathrm{a}\mathrm{r}\mathrm{e}\mathrm{c}\mathrm{e}\mathrm{l}\mathrm{l}\left(1\right)\times \mathrm{D}\mathrm{i}\mathrm{l}\mathrm{u}\mathrm{t}\mathrm{i}\mathrm{o}\mathrm{n}\mathrm{f}\mathrm{a}\mathrm{c}\mathrm{t}\mathrm{o}\mathrm{r}\left(40\right)}{\mathrm{V}\mathrm{o}\mathrm{l}\mathrm{u}\mathrm{m}\mathrm{e}\mathrm{f}\mathrm{a}\mathrm{c}\mathrm{t}\mathrm{o}\mathrm{r}\left(0.1\right)}$ $\mathrm{M}\mathrm{C}\mathrm{H}\left(\mathrm{p}\mathrm{g}\right)=\frac{\mathrm{H}\mathrm{b}\times 10}{\mathrm{R}\mathrm{B}\mathrm{C}}$