Flow cytometry analysis of B7H6 expression on tumor cells were performed by staining cells with anti-B7H6 mAbs,8 (link) followed by DyLight 649-conjugated goat anti-mouse IgG (clone: Poly4053, Biolegend, San Diego, CA). For staining of mouse cells, the samples were pre-incubated with FcR block antibody (anti-mouse CD16/CD32, 2.4G2; Bio X Cell, Lebanon, NH), and for staining of human cells, samples were pre-incubated with human gamma globulin (Cohn fraction, Sigma-Aldrich). Samples were analyzed by Accuri C6 cytometer (BD Biosciences, San Jose, CA) and data was analyzed by Accuri or FlowJo software (TreeStar Inc, Ashland, OR).
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B7H6 Expression Analysis by Flow Cytometry
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Corresponding Organization :
Other organizations : Dartmouth College, Dartmouth–Hitchcock Medical Center
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Variable analysis
independent variables
- Staining cells with anti-B7H6 mAbs
- B7H6 expression on tumor cells
- Pre-incubation of mouse cells with FcR block antibody (anti-mouse CD16/CD32, 2.4G2)
- Pre-incubation of human cells with human gamma globulin (Cohn fraction, Sigma-Aldrich)
- Positive control: Staining with anti-B7H6 mAbs, followed by DyLight 649-conjugated goat anti-mouse IgG
- Negative control: Not specified
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