Cytokine Profiling of Transmigrated Cells

TEM assays were performed as above for 96 hours, the transmigrated cells were expanded, and protein extracted as described previously [26 (link)]. Protein was loaded and analyzed on a standard human cytokine array membrane (ARY005, R&D Systems) as per the manufacturer’s protocol.

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Crawford J.R., Trial J., Nambi V., Hoogeveen R.C., Taffet G.E, & Entman M.L. (2016). Plasma Levels of Endothelial Microparticles Bearing Monomeric C-reactive Protein Are Increased in Peripheral Artery Disease. Journal of cardiovascular translational research, 9(3), 184-193.

Publication 2016

ARY005

Assays Cells Cytokine Human Membrane Protein

Corresponding Organization :

Other organizations : Baylor College of Medicine, Houston Methodist, Methodist Hospital, Michael E. DeBakey VA Medical Center

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Variable analysis

independent variables

Duration of TEM assays (96 hours)

dependent variables

Cytokine levels in transmigrated cells

control variables

TEM assay protocol
Protein extraction and analysis methods

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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