The efficacy of wtSPIDAR and mSPIDAR to dissociate the binding of SARS-CoV-2 spike S1 with ACE2 was investigated using the ACE2:SARS-CoV-2 Spike inhibitor screening assay kit (BPS Bioscience, San Diego, CA) according to manufacturer’s instructions as described by us recently (11 (link)). Briefly, 96-well nickel-treated plate provided by the manufacturer was coated with ACE2 solution. After washing with immuno buffer and treatment with blocking buffer, different concentrations of wtSPIDAR and mSPIDAR were added to each well followed by addition of SARS-CoV-2 Spike (RBD)-Fc. After washing and incubation with blocking buffer, plates were treated with anti-mouse Fc-HRP followed by addition of an HRP substrate. Resultant chemiluminescence was monitored in a Perkin Elmer multimode microplate reader, Victor X5.
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ACE2-SARS-CoV-2 Spike Inhibitor Screening
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Corresponding Organization :
Other organizations : Rush University Medical Center, Northwestern University, Jesse Brown VA Medical Center
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Variable analysis
independent variables
- WtSPIDAR concentration
- MSPIDAR concentration
- Dissociation of the binding of SARS-CoV-2 spike S1 with ACE2
- ACE2 solution coated on the 96-well nickel-treated plate
- Immuno buffer used for washing
- Blocking buffer used for treatment
- SARS-CoV-2 Spike (RBD)-Fc
- Anti-mouse Fc-HRP
- HRP substrate
- Not specified
- Not specified
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