Total RNAs were isolated using the Isogen II (NipponGene). The sizes of cellular and exosomal RNAs collected from the PK-45H cells were examined using the Agilent 2100 Bioanalyzer and Agilent RNA 6000 Pico Kits (Agilent Technologies)36 (link). Total RNAs from HUVECs treated with exosomes were subjected to microarray analysis, as described below.
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