DNA was isolated from cells using the QIAmp system (Qiagen). The concentration of DNA was estimated using the NanoDrop ND-1000 spectrophotometer. DNA quality was assessed using Agilent 2100 Bioanalyser (Agilent Technologies, Santa Clara, CA, USA). PCR primer sequences for IGHV gene framework region 3 (FR3) were designed as previously reported (Kummalue et al, 2010 (link)). PCR of the IGH genes to assess clonality used the BIOMED-2 system. Master mixes were purchased from Invivoscribe Technologies (San Diego, CA, USA), and the PCR was done as per manufacturer’s instructions and used HotStart Taq DNA polymerase (Qiagen) (Burack et al, 2010 (link)).
Protocol detail
Immunoglobulin Gene Clonality Assessment
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : AdventHealth Orlando
Other organizations : University of Michigan–Ann Arbor, The University of Texas MD Anderson Cancer Center, Baylor College of Medicine
Top 5 similar protocols
Variable analysis
independent variables
- PCR primer sequences for IGHV gene framework region 3 (FR3)
- DNA concentration
- DNA quality
- IGH gene clonality
- DNA isolation using the QIAmp system (Qiagen)
- DNA concentration estimation using the NanoDrop ND-1000 spectrophotometer
- DNA quality assessment using Agilent 2100 Bioanalyser (Agilent Technologies, Santa Clara, CA, USA)
- PCR of the IGH genes using the BIOMED-2 system
- Master mixes purchased from Invivoscribe Technologies (San Diego, CA, USA)
- PCR using HotStart Taq DNA polymerase (Qiagen)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!