Nanoparticle Characterization Techniques

Nanoparticle maximum absorption wavelengths were obtained using the Varian Cary 50 Bio UV-Visible Spectrometer (McKinley Scientific). Nanoparticle size and zeta potential measurements were obtained using the Zeta-Sizer Nanoseries ZS90 (Malvern Instruments, Worcestshire, UK). Hydrodynamic size based upon Brownian motion was measured in phosphate buffered saline (PBS) solution using DLS (dynamic light scattering). Shape and size were also previously determined using scanning electron microscope (SEM), with the presence of lipids on the particle cores confirmed using a Fourier transform infrared (FTIR) spectroscopy [18 (link)].

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England C.G., Huang J.S., James K.T., Zhang G., Gobin A.M, & Frieboes H.B. (2015). Detection of Phosphatidylcholine-Coated Gold Nanoparticles in Orthotopic Pancreatic Adenocarcinoma using Hyperspectral Imaging. PLoS ONE, 10(6), e0129172.

Publication 2015

Zeta-Sizer Nanoseries ZS90

Fourier transform infrared spectroscopy Hydrodynamic Lipids Phosphate Saline solution Scanning electron microscope

Corresponding Organization : University of Louisville

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Variable analysis

independent variables

Nanoparticle maximum absorption wavelengths
Nanoparticle size
Nanoparticle zeta potential

dependent variables

Hydrodynamic size based upon Brownian motion
Nanoparticle shape and size

control variables

Phosphate buffered saline (PBS) solution

controls

No positive or negative controls were explicitly mentioned.

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