Measurement of Physiological Parameters in Mice

Measurement of core body temperature and heart rate was performed using a radio-telemetry transmitter (TA11ETA-F10; DataSciences International, St Paul, MN, USA) and Dataquest A.R.T. 4.30 (DataSciences International) according to a method^{5 (link)}. Briefly, a radio-telemetry transmitter was implanted following the surgical procedure described by the manufacturer. After the surgery, the mice were allowed to recover for ~10 days before testing. On the test day, each mouse was placed in a separate test cage (17.5 × 10.5 × 15 cm) without a lid placed in a chemical fume hood, habituated for 10 min, and subjected to presentation of filter paper containing test compound (Fig. 1b, e and Supplementary Fig. 1).
For the restrained condition, mice were implanted with radio-telemetry probes approximately 10 days prior to the experiment, as described above. On the test day, each mouse was placed in a separate test cage (17.5 × 10.5 × 15 cm) without a lid, allowed to habituate for 10 min, and physiological parameters were analyzed for 10 min. Immediately afterward, mice were restrained in ventilated 50-ml plastic tubes (Becton Dickinson and Company, Franklin Lakes, NJ, USA), and physiological parameters were analyzed for 30 min (Fig. 1d, f).
Physiological parameters were automatically transmitted from the device every 10 s using Dataquest A.R.T. software (DataSciences International).