Urinary Protein and Kidney Function Assays

Urinary total protein (Pyrogallol red-molybdate method) was measured by 7180 automatic biochemical analyzer (Hitachi). For proteinuria, there was 1 missing value in patient with negative serum anti-PLA2R antibody and positive glomerular PLA2R deposition. Serum total protein (Biuret method), serum albumin (Bromcresol green method), serum creatinine (Jaffe method), and serum urea (Urease methods) were measured by Synchron DXC800 automatic biochemical analyzer (Beckman Coulter). Estimated glomerular filtration rate (eGFR) levels were calculated using the Chronic Kidney Disease Epidemiology Collaboration equation recommended by the Kidney Disease Improving Global Outcomes.^{[9 (link)]}