Cells were seeded at a density of 1 × 10 3 cells/well in 96-well plates and treated with 0.2% FBS, 10–100 µM galunisertib (MedChemExpress, Monmouth Junction, NJ, USA), or vehicle (10% FBS and 0.2% DMSO (Dimethyl sulfoxide), as a control) for 48, 72, or 96 h. Cell viability was evaluated using the WST-1 assay (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt, Roche Diagnostics, Basel, Switzerland) for mitochondrial dehydrogenase activity, as previously described [12 (link)].
Free full text: Click here
