Comprehensive Mouse Metabolic Phenotyping

Body weight and composition was determined weekly using quantitative magnetic resonance (QMR) (Bruker's Minispec MQ10, Housten Texas, USA) as described [3] (link). Energy expenditure of single mice was measured by indirect calorimetry over 24 h using an open respirometric system at 11 week of age as described before [2] (link). Mice were kept in normal housing cages and had free access to food and water during the measurement. Locomotor activity was assessed by parallel measurement of spontaneous physical activity using an infrared method and running wheel activity providing mice continuous voluntary access to a running wheel counting wheel rotations (both TSE Systems GmbH, Germany) in the same cages.

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Ost M., Werner F., Dokas J., Klaus S, & Voigt A. (2014). Activation of AMPKα2 Is Not Crucial for Mitochondrial Uncoupling-Induced Metabolic Effects but Required to Maintain Skeletal Muscle Integrity. PLoS ONE, 9(4), e94689.

Publication 2014

Minispec MQ10

Body weight Energy expenditure Food Indirect calorimetry Locomotor activity Magnetic resonance Mice

Corresponding Organization :

Other organizations : German Institute of Human Nutrition

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Variable analysis

independent variables

Body weight and composition measurement by quantitative magnetic resonance (QMR)
Energy expenditure measurement by indirect calorimetry
Locomotor activity assessment by infrared method and running wheel activity

dependent variables

Body weight and composition
Energy expenditure
Locomotor activity

control variables

Mice kept in normal housing cages
Mice had free access to food and water during the measurement

controls

No positive or negative controls were explicitly mentioned in the input.

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