LacY-eYFP expressing E. coli C41 cells were grown overnight in LB medium with appropriate antibiotics and subsequently diluted 1000 times in EZ rich medium (Teknova) with 0.4% glycerol. When the cell culture reached an OD600 of 0.3, Lacy-eYFP expression was induced with 0.01%(v) L-arabinose for 30 minutes at 37 °C. The cells were centrifuged at 3000 rcf for 5 minutes and resuspended in EZ rich medium with 0.2% glucose. 3 μL of the bacterial culture was transferred onto a microscope coverslide and covered by an agarose gel pad.
Images were captured with an exposure time of 50 ms using the open source software μManager [92 (link), 93 ].
To obtain the final STORM reconstruction, 2000 frames were collected and processed with the ImageJ plugin ThunderSTORM [68 (link)].
All data and code used to generate the figures in this article can be found atZenodo (DOI: 10.5281/zenodo.2637790 ) and GitHub , respectively.
Images were captured with an exposure time of 50 ms using the open source software μManager [92 (link), 93 ].
To obtain the final STORM reconstruction, 2000 frames were collected and processed with the ImageJ plugin ThunderSTORM [68 (link)].
All data and code used to generate the figures in this article can be found at
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