Drosophila Neuronal Cell Labeling and Imaging

The following stocks were employed - y[1] w[*]; Mi{y[+mDint2]=MIC}Syt1[MI02197] (BDSC#35973) (Venken et al., 2011 (link)), y(1) w(*) Mi(y[+mDint2]=MIC) Sh(MI10885) (BDSC#56260), y(1) w(*);Mi(y[+mDint2]=MIC)Shal(MI10881) (BDSC#56089) (Venken et al., 2011 (link)), y(1) w(*); Mi(y[+mDint2]=MIC) Shab(MI00848) (BDSC#34115) (Venken et al., 2011 (link)), nSyb-GAL4(GMR57C10)(BDSC#39171) (Pfeiffer et al., 2008 (link)), repo-GAL4 (BDSC#7415) (Sepp et al., 2001 (link)), repo-QF2 (BDSC#66477) (Lin and Potter, 2016 (link)), Shaw-GAL4 (BDSC#60325) (Venken et al., 2011 (link); Li-Kroeger et al., 2018 (link)), Ddc-GAL4(BDSC#7009) (Feany and Bender, 2000 (link)), TH-GAL4(BDSC#8848) (Friggi-Grelin et al., 2003b (link)), Trh-GAL4(BDSC#38389) (Alekseyenko et al., 2010 (link)), UAS_H2A-GFP (Sherer et al., 2020 (link)), QUAS_H2B-mCherry (Sherer et al., 2020 (link)), brp-GAL4 (this manuscript), UAS_H2A::GFP-T2A-mKok::Caax (this manuscript). All lines were raised on standard media at 25°C, 50% RH.

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Jiao W., Spreemann G., Ruchti E., Banerjee S., Vernon S., Shi Y., Stowers R.S., Hess K, & McCabe B.D. (2022). Intact Drosophila central nervous system cellular quantitation reveals sexual dimorphism. eLife, 11, e74968.

Publication 2022

nSyb-GAL4 repo-GAL4 repo-QF2 Ddc-GAL4 TH-GAL4 Trh-GAL4

Syt1

Corresponding Organization :

Other organizations : École Polytechnique Fédérale de Lausanne, Montana State University

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Variable analysis

independent variables

Y[1] w[*]
Mi{y[+mDint2]=MIC}Syt1[MI02197] (BDSC#35973)
Y(1) w(*) Mi(y[+mDint2]=MIC) Sh(MI10885) (BDSC#56260)
Y(1) w(*);Mi(y[+mDint2]=MIC)Shal(MI10881) (BDSC#56089)
Y(1) w(*); Mi(y[+mDint2]=MIC) Shab(MI00848) (BDSC#34115)
NSyb-GAL4(GMR57C10)(BDSC#39171)
Repo-GAL4 (BDSC#7415)
Repo-QF2 (BDSC#66477)
Shaw-GAL4 (BDSC#60325)
Ddc-GAL4(BDSC#7009)
TH-GAL4(BDSC#8848)
Trh-GAL4(BDSC#38389)
UAS_H2A-GFP
QUAS_H2B-mCherry
Brp-GAL4
UAS_H2A::GFP-T2A-mKok::Caax

dependent variables

Not explicitly mentioned

control variables

All lines were raised on standard media at 25°C, 50% RH.

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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