Immunohistochemistry Protocol for Protein Detection

The protocol of IHC was described previously [36 (link)]. Sections were prepared in 10 μm. The primary antibodies against β-catenin (Abcam, #ab16051, 1:1000), Ki67 (Abcam, #ab245113, 1:1000), PDGFD, E-cadherin (Abcam, #ab1416, 1:500), and N-cadherin (Abcam, #ab76057, 1:1000) were used. Following PBS washes, secondary antibodies (1:1000 was applied to sections. Lastly, the signal was developed by DAB and validated by two experienced pathologists.

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Yao J, & Liu X. (2023). lncRNA PITPNA-AS1 promotes cell proliferation and metastasis in hepatocellular carcinoma by upregulating PDGFD. Aging (Albany NY), 15(10), 4071-4083.

Publication 2023

ab16051 ab245113 ab1416 ab76057

Antibodies E cadherin N cadherin Pathologists β catenin

Corresponding Organization :

Other organizations : Yinchuan First People's Hospital

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Variable analysis

independent variables

Primary antibodies against β-catenin (Abcam, #ab16051, 1:1000), Ki67 (Abcam, #ab245113, 1:1000), PDGFD, E-cadherin (Abcam, #ab1416, 1:500), and N-cadherin (Abcam, #ab76057, 1:1000)

dependent variables

Signal developed by DAB

control variables

Sections were prepared in 10 μm
Following PBS washes, secondary antibodies (1:1000) were applied to sections

controls

Validated by two experienced pathologists

Annotations

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