Fatty Acid Profiling of Silage and Algae

The fatty acids in corn silage, haylage, and the algal blend were transesterified by acid-catalyzed methylation (20 (link)) using nonadecanoic acid (C19:0, Matreya LLC, State College, PA, USA) as the internal standard. The resulting fatty acid methyl esters were analyzed using a Shimadzu GC-2010 Plus gas chromatograph (Shimadzu Europe GmbH, Duisburg, Germany) equipped with a capillary column (Omegawax 250, 30 m × 0.25 mm internal diameter, 0.25 μm film thickness; Supelco, Bellefonte, PA, USA) and a flame ionization detector, as described by Mota et al. (21 (link)). The identification of fatty acids was done by comparison with commercial standards (Supelco 37 Component FAME Mix, BAME Mix, PUFA No. 1, PUFA No. 2, PUFA No. 3, Sigma-Aldrich Co. LLC; GLC-110 Mixture, Matreya LLC) and quantified based on the internal standard (C19:0). Analyses were run in duplicate.