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Drosophila Neuronal Genetic Toolkit

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All flies were raised on standard food at 25°C with a 12-hour light:12-hour dark cycle, except for the Tubulin-Gal80^ts experiments to induce expression at different developmental stages, where flies were raised at either 18° or 29°C. Male and female flies were collected at eclosion and aged as specified before performing experiments. VT030559-GAL4 was obtained from the Vienna Drosophila Resource Center stock center. VAChT^[MI08244] (#55439), nsyb-GAL4 (#51941), VGluT-GAL4 (#60312), VGluT-p65-AD (#82986), VGluT-GAL4-DBD (#60313), ChAT-GAL4 (#60317), GH146-GAL4 (#30026), GMR81C04-GAL4 (#48378), VGAT-GAL4 (#84696), UAS-miR-190-sponge (#61397), UAS-scramble-sponge (#61501), UAS-Flp (#4539), UAS-CD4-GFP^1–10 (#93016), VGluT-Gal80 (#58448), and tubulin-Gal80^ts (#7016) were obtained from Bloomington Drosophila Stock Center. UAS-myrGFP-2A–RedStinger (27 (link)) was obtained from the Ganetzky laboratory at University of Wisconsin, and UAS-UNC84::GFP was from G. Henry at Janelia Research Campus. Table S1 lists all the lines used in this study with their source and whether their mRNA contains the native gene’s 3′UTR. Table S2 lists the full genotypes of all lines used in this study by figure panel.

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Chen N., Zhang Y., Rivera-Rodriguez E.J., Yu A.D., Hobin M., Rosbash M, & Griffith L.C. (2023). Widespread posttranscriptional regulation of cotransmission. Science Advances, 9(22), eadg9836.

Publication 2023

nsyb-GAL4 VGluT-GAL4 ChAT-GAL4 GH146-GAL4 VGAT-GAL4 UAS-Flp tubulin-Gal80ts

Drosophila Female Flies Food Gene Genotypes Light Male Mrna Sponge Tubulin

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Variable analysis

independent variables

Developmental stage to induce expression (using Tubulin-Gal80^{ts}): 18°C or 29°C

dependent variables

Not explicitly mentioned

control variables

All flies were raised on standard food at 25°C with a 12-hour light:12-hour dark cycle
Male and female flies were collected at eclosion and aged as specified before performing experiments

controls

Positive control: UAS-scramble-sponge
Negative control: Not explicitly mentioned

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