Isolation of Stromal Vascular Fraction

The isolation of SVF cells was done by a previously described methodology, with slight modifications^{14 (link),17 (link)}. Briefly, 1–2 g of SAT or MAT were incubated with Hanks’ balanced salt solution supplemented with 10 mM HEPES (both from Sigma-Aldrich), 4% BSA (Biowest), and 0.125 mg/mL Liberase™ TL Research Grade (Roche Diagnostics, Risch-Rotkreuz, Switzerland) in a water bath at 37 °C during 60–90 min with manual agitation every 10 min. Digested samples were then passed through a 100-μm cell strainer and centrifuged at 280×g for 10 min at 4 °C. The pellet, corresponding to the SVF cells, was resuspended in complete RPMI medium.