A previously described protocol for data acquisition was followed (25 (link)). Briefly, isotope enrichment data were collected on a Cameca NanoSIMS 50L housed in the Center for Microanalysis at the California Institute of Technology. Six masses were collected corresponding to the 1H−, 2H−, 12C−, 13C−, 14N12C−, and 15N12C− ions, for the determination of 2H/1H, 13C/12C, and 15N/14N ratios, respectively, using a tuning similar to that described by Kopf et al. (52 (link)).
Jiménez Otero F., Chadwick G.L., Yates M.D., Mickol R.L., Saunders S.H., Glaven S.M., Gralnick J.A., Newman D.K., Tender L.M., Orphan V.J, & Bond D.R. (2021). Evidence of a Streamlined Extracellular Electron Transfer Pathway from Biofilm Structure, Metabolic Stratification, and Long-Range Electron Transfer Parameters. Applied and Environmental Microbiology, 87(17), e00706-21.
Tuning parameters similar to that described by Kopf et al. (52 (link))
controls
No positive or negative controls were explicitly mentioned.
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