Transgenic lines 3D7-150HA and D37-101HA were generated by transfecting P. falciparum strain 3D7 with plasmids pPTEX150-HA/Str3' or pHSP101-HA/Str3' respectively. Parasites containing integrated forms of the plasmid were obtained by repeated drug cycling in the presence and absence of WR99210. Antibodies were also raised against recombinant hexa-his fusion proteins derived from either PTEX150 (amino acids 181-236) or HSP101 (amino acids 68-170). These transgenic lines and antibodies, along with other antibodies described in this study, were used in immunoprecipitation experiments as well as western blot and immunofluoresence analysis. Immunoprecipitations were performed on mixed ring and trophozoite-stage infected erythrocytes in which the erythrocyte had been permeabilised with tetanolysin or first cross-linked with 2mM DSP followed by saponin lysis. Proteins present in parasite lysates (generated by incubation of the parasites on ice in 1% (v/v) Triton-X 100 or RIPA buffer) that were pulled down with anti-HA antibodies or other antibodies described in this study were identified either by LC-MS/MS based sequencing or by Western blot analysis. For IFA, parasites were fixed with ice-cold 90% acetone/10 % methanol prior to incubation with various primary and secondary antibody combinations.