Neuroprotective Effects of Aluminum Chloride, Valsartan, and Sacubitril/Valsartan in Rats

Aluminum chloride powder, valsartan (Amoun Pharmaceuticals & Chemical
Industries), sacubitril/valsartan combination; 97 mg/103 mg (Novartis), all
chemicals were dissolved in distilled water. Kits: Amyloid beta
(Aβ1–42): ELISA for rat brain (Leader Trade Company) & Thiobarbituric Acid
Reactive Substances (TBARS) Assay (MDA Quantitation) (Oxiselect Company).
Animals used: In this study, 72 male adult Wistar albino rats of matching age and
weight (200–230 g) were used. Animals were housed in two a cage and were kept
under hygienic conditions at a room temperature of 22°C and 55% humidity with a
12-h light/12-h dark schedule. Rats were fed standard rat pellets, and water was
freely provided. This study was approved by Cairo University’s Institutional
Animal Care and Use Committee (IACUC), medical sciences sector. The experiments
were carried out in accordance with the ethical guidelines of the animal welfare
application number (CU/III/F/28/19).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

El-din Hussein A.S., Abou-El Nour R.K., Khorshid O.A, & Osman A.S. (2023). Study of the possible effect of sacubitril/valsartan combination versus valsartan on the cognitive function in Alzheimer’s disease model in rats. International Journal of Immunopathology and Pharmacology, 37, 03946320231161469.

Publication 2023

valsartan sacubitril

Adult Albino Aluminum chloride Amyloid Animal Assay Brain Elisa Humidity Light Male Pellets Pharmaceuticals Powder Sacubitril valsartan Valsartan Wistar rats

Corresponding Organization : Cairo University

Top 5 similar protocols

Variable analysis

independent variables

Aluminum chloride powder
Valsartan (Amoun Pharmaceuticals & Chemical Industries)
Sacubitril/valsartan combination; 97 mg/103 mg (Novartis)

dependent variables

Amyloid beta (Aβ1–42) levels in rat brain (measured using ELISA)
Thiobarbituric Acid Reactive Substances (TBARS) levels (measured using MDA Quantitation)

control variables

Rat age and weight (200–230 g)
Rat housing conditions (two per cage, hygienic conditions, 22°C, 55% humidity, 12-h light/12-h dark schedule)
Rat diet (standard rat pellets, free access to water)

positive controls

None specified

negative controls

None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!