(Miltenyi). 1×106 T cells cultured with platebound
α-CD3 (2C11) and α-CD28 (37.51) from BioXcell at 1μg/mL
each for 48 or 66 hrs. Inhibitors purchased and used as follows: ZSTK474
(1μM, Sigma), Rapamycin (10nM, Selleckchem), NFAT inhibitor (10μM,
Tocoris), Mek inhibitor PD0325901 (100nM, Peprotech) and Erk inhibitor FR180204
(10μM, Tocoris). In vitro TR suppression
assays were performed as previous described (20 (link)). Chemotaxis assay performed as previously described (21 (link)).