HEK293 Cell Culture and Transfection
Corresponding Organization : University of Toronto
Other organizations : Mila - Quebec Artificial Intelligence Institute, University of Eastern Finland, Lunenfeld-Tanenbaum Research Institute, Sinai Health System
Variable analysis
- Transfection with NanoLuc plasmids using linear polyethylenimine 25 kDa (PEI 25 K)
- Cell pellets
- Growth of HEK293 cells in DMEM (Wisent, 319-005-CL) supplemented with 10% fetal calf serum (Sigma, F1051) at 37 °C under 5% CO2
- Seeding of cells into 15 cm plates
- No positive or negative controls were explicitly mentioned.
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