HEK293 cells were grown in DMEM (Wisent, 319-005-CL) supplemented with 10% fetal calf serum (Sigma, F1051) at 37 °C under 5% CO2. Cells were seeded into 15 cm plates and co-transfected with NanoLuc plasmids using linear polyethylenimine 25 kDa (PEI 25 K; Polysciences, 23966). Cells were harvested after 24 h and cell pellets were stored at − 80 °C.
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