Mice were bred at UCLA and Memorial Sloan Kettering Cancer Center in accordance with the guidelines of the institutional Animal Care and Use Committee (IACUC). The following mouse strains were used this study: C57BL/6 (CD45.2) (Jackson Labs, #000664), B6.SJL (CD45.1) (Jackson Labs, #002114), Rag2−/− (Jackson Labs #008449), Klra8−/− (Ly49H-deficient), Zbtb32−/−, and Il12rb2−/−x Il18ra1−/− (MSKCC, J.C. Sun). Experiments were conducted using 7–8 week old age- and gender-matched mice in accordance with approved institutional protocols.
Generation of mixed bone marrow chimeric mice was performed as previously described18 (link). Parabiosis surgery was performed as previously described12 (link), 15 (link), 16 (link), 17 (link). Intravascular labeling of lymphocytes of experimental mice was performed by injecting (i.v.) 2.5 μg of fluorophore-conjugated CD45 (30-F11) and euthanized 3 minutes later.
Generation of mixed bone marrow chimeric mice was performed as previously described18 (link). Parabiosis surgery was performed as previously described12 (link), 15 (link), 16 (link), 17 (link). Intravascular labeling of lymphocytes of experimental mice was performed by injecting (i.v.) 2.5 μg of fluorophore-conjugated CD45 (30-F11) and euthanized 3 minutes later.
