Culturing Pigmented Melanoma and Expression Cells

B16F10 cells were purchased from ATCC. Apigmented B16F10 cells used for imaging were generated by genetic deletion of Tyrosinase-related-protein-2 (TRP2), referred to as B16F10-Trp2 KO cells (83 (link)). Tumor cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, ATCC) supplemented with 10% Fetal Bovine Serum (FBS, Gibco). FreeStyle 293-F cells and Expi293 cells were purchased from Invitrogen and cultured in FreeStyle expression medium (Gibco) and Expi293 expression medium (Gibco), respectively. CHO DG44 cells were cultured in ProCHO5 (Lonza) supplemented with 4 mM L-glutamine, 0.1 mM hypoxanthine, and 16 μM thymidine. Tumor cells were maintained at 37°C and 5% CO₂ and FreeStyle 293-F cells, Expi293 cells, and CHO DG44 cells were maintained at 37°C and 8% CO₂. All cells tested negative for mycoplasma contamination.