X-InDel Genotyping Protocol for Unrelated Samples

All blood samples were amplified directly without DNA extraction. The 268 unrelated samples were genotyped using our new established multiplex amplification system on the Thermo 96-Well PCR System (Thermo Fisher Scientific Company, Carlsbad, United States). The analyzed panel including 38 X-InDel markers, namely rs10671504, rs11277082, rs1160845, rs143123845, rs149102585, rs16367, rs16368, rs16397, rs16637, rs17394, rs199731653, rs2307707, rs2307741, rs2308033, rs2308280, rs25581, rs3048996, rs3077884, rs3215490, rs34763847, rs35574346, rs35954471, rs363794, rs4030406, rs45449991, rs56820033, rs57608175, rs57843641, rs58595330, rs59605609, rs60283667, rs71671860, rs3216913, rs10699224, rs3859989, rs61260787, rs36094418 and rs79829945. The localizations of the different markers were previously described in our published article (Chen et al., 2021 (link)). Separation of PCR-amplified products were performed on the ABI 3130xL DNA Analyzer (Applied Biosystems, Foster City, CA, United States). Electropherogram analysis and allele assignment were performed with GeneMapper v 4.0.

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Zhang L., Zhu Z., Du W., Li S, & Liu C. (2022). Genetic Structure and Forensic Feature of 38 X-Chromosome InDels in the Henan Han Chinese Population. Frontiers in Genetics, 12, 805936.

Publication 2021

Thermo 96-Well PCR System ABI 3130xL DNA Analyzer

Allele Blood Indel Multiplex pcr

Corresponding Organization : Xi'an Jiaotong University

Other organizations : Xinxiang Medical University, Gene Tech (China)

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Genotypes of 38 X-InDel markers in 268 unrelated samples

control variables

Amplification of blood samples directly without DNA extraction
Use of the Thermo 96-Well PCR System for multiplex amplification
Separation of PCR-amplified products on the ABI 3130xL DNA Analyzer
Electropherogram analysis and allele assignment using GeneMapper v 4.0

positive controls

None mentioned

negative controls

None mentioned

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