We have used a mouse model of partial “warm” hepatic IRI (2 (link)). In brief, animals were anesthetized, injected with heparin (100U/kg i.p.), and the arterial/portal venous blood supply to the cephalad lobes was interrupted by an atraumatic clip for 90min. Sham-operated mice underwent the same procedure, but without vascular occlusion. In the treatment groups, animals were infused 1h prior to the onset of liver ischemia with a single dose of PACAP27 or PACAP38 neuropeptide (50nmol/mouse i.v., Phoenix Pharmaceuticals, Burlingame, CA) dissolved in PBS. Some recipients were given H-89 (cAMP-PKA inhibitor; 20nmol/mouse i.v., Sigma-Aldrich, St. Louis, MO) dissolved in dimethyl sulfoxide (DMSO). Mice were sacrificed at various time-points of reperfusion; liver and serum samples were collected for analysis.
Protocol detail
Hepatic Ischemia-Reperfusion Injury in Mice
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Animals
Arterial
Clip
Dimethyl sulfoxide
Heparin
Ischemia
Liver
Mice
Neuropeptide
Occlusion
Pacap27
Pacap38
Pharmaceuticals
Pka inhibitor
Portal venous
Reperfusion
Serum
Vascular
Corresponding Organization :
Other organizations : University of California, Los Angeles, Zhejiang University
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Variable analysis
independent variables
- Infusion of PACAP27 neuropeptide (50nmol/mouse i.v.)
- Infusion of PACAP38 neuropeptide (50nmol/mouse i.v.)
- Infusion of H-89 (cAMP-PKA inhibitor; 20nmol/mouse i.v.)
- Liver samples
- Serum samples
- Anesthesia
- Heparin injection (100U/kg i.p.)
- Atraumatic clip for 90min to interrupt arterial/portal venous blood supply to the cephalad lobes
- Sham-operated mice underwent the same procedure, but without vascular occlusion
- Positive control: Sham-operated mice
- Negative control: Not explicitly mentioned
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