orco ZFNs were generated using the CompoZr® Custom ZFN Service (Sigma-Aldrich Life Science, St. Louis, MO, USA). Of the 16 ZFN pairs screened, the orco exon 1 ZFN pair used in this study had the greatest activity, comparable to a highly active positive control ZFN pair that targets CCR5. Mutant alleles were detected by Illumina sequencing of an amplicon that contained the ZFN cut site. Mutant alleles were isolated using size-based genotyping of amplicons surrounding the deletion site, allowing us to discriminate heterozygous from homozygous individuals. Mosquitoes were tested for their response to odour cues in a modified version of a Gouck two-port olfactometer19 (link).
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Generation and Evaluation of orco ZFN Mutants
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Corresponding Organization :
Other organizations : Rockefeller University, University of California, Irvine, Howard Hughes Medical Institute
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Variable analysis
independent variables
orco ZFNs
- Mutant alleles detected by Illumina sequencing
- Mosquito response to odour cues in a modified version of a Gouck two-port olfactometer
- Not explicitly mentioned
- Positive control: A highly active ZFN pair that targets CCR5
- Negative control: Not specified
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