Echocardiography and Hemodynamics in Rats

Four weeks after the intraperitoneal administration of IMD1-53, all rats were lightly anesthetized with 1% sodium pentobarbital at 3 mg/100 mg (Sinopharm Chemical Reagent Co., Ltd.), and transthoracic echocardiography was performed with a 30 MHz high-frequency transducer (Vevo2100; VisualSonics, Inc., Toronto, ON, Canada). To calculate the left-ventricular ejection fraction (LVEF) and left-ventricular fractional shortening (LVFS), the left-ventricular diameter at the end of diastole and end of systole was measured using M-mode tracing [21 (link),22 (link)]. After the echocardiographic study, the hemodynamic parameters were measured using the BL-420E biological system (Chengdu Tai-Meng Science and Technology Co., Ltd., Chengdu, Sichuan, China) [20 (link)]. Briefly, a catheter was inserted into the left ventricle through the right common carotid artery to record the left-ventricular end-diastolic pressure (LVEDP) [23 (link)].

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Ma S., Yan F, & Hou Y. (2023). Intermedin 1-53 Ameliorates Atrial Fibrosis and Reduces Inducibility of Atrial Fibrillation via TGF-β1/pSmad3 and Nox4 Pathway in a Rat Model of Heart Failure. Journal of Clinical Medicine, 12(4), 1537.

Publication 2023

sodium pentobarbital Vevo2100

Biological Catheter Common carotid artery Diastole Diastolic pressure Echocardiographic Hemodynamic Imd1 Intraperitoneal administration Left ventricular Rats Sodium pentobarbital Systole Transducer Ventricular ejection fraction

Corresponding Organization : Qilu Hospital of Shandong University

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Variable analysis

independent variables

Intraperitoneal administration of IMD1-53

dependent variables

Left-ventricular ejection fraction (LVEF)
Left-ventricular fractional shortening (LVFS)
Left-ventricular end-diastolic pressure (LVEDP)

control variables

1% sodium pentobarbital at 3 mg/100 mg used for light anesthesia
30 MHz high-frequency transducer (Vevo2100) used for transthoracic echocardiography
BL-420E biological system used for measuring hemodynamic parameters

controls

No positive or negative controls were explicitly mentioned in the provided information.

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