TaELP2 Gene Silencing via BSMV-Mediated RNA Interference

The cDNA sequences of TaELP2 were analyzed using SiFi12 software, and two segments with high and specific siRNA numbers were selected for gene silencing, TaELP2-as1 and TaELP2-as2, respectively. Specific primers (Table S10) were designed, and two specific cDNA fragments of TaELP2 were obtained using PCR and constructed into BSMV:γ genome. Then, the seedlings (Yannong 19) at the two-leaf stage were inoculated using friction. After 10 days of inoculation, the virus phenotype of the leaves was observed. The plants with virus phenotype were identified with RT-qPCR and treated in dark. The leaf phenotype and chlorophyll content were observed at 9 days and 14 days after treatment. The determination method of chlorophyll content was from Porra et al. [84 (link)].

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Guo F., Islam M.A., Lv C., Jin X., Sun L., Zhao K., Lu J., Yan R., Zhang W., Shi Y., Li N, & Sun D. (2023). Insights into the Bioinformatics and Transcriptional Analysis of the Elongator Complexes (ELPs) Gene Family of Wheat: TaELPs Contribute to Wheat Abiotic Stress Tolerance and Leaf Senescence. Plants, 12(4), 952.

Publication 2023

After treatment Cdna Chlorophyll Friction Genome Inoculation Leaf Phenotype Primers Seedlings Sirna Virus Virus plants

Corresponding Organization : Shanxi Agricultural University

Other organizations : University of North Texas

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Variable analysis

independent variables

Presence of virus: BSMV:γ genome containing TaELP2-as1 or TaELP2-as2

dependent variables

Virus phenotype of the leaves
Leaf phenotype
Chlorophyll content

control variables

Plant variety: Yannong 19
Plant growth stage: Two-leaf stage
Time points for observations: 9 days and 14 days after treatment

controls

Positive control: Not mentioned
Negative control: Not mentioned

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