DU145 Cell Line Characterization

DU145 cell line was obtained from the American Tissue Type Collection (ATCC, Manassas, VA). The cell line was cultured in Iscove’s modified Dulbecco’s medium (IMDM) from Mediatech (Manassas,VA) supplemented with 10% fetal bovine serum (FBS) Hyclone Laboratories (Novato, CA) and incubated at 37° in a 5% CO₂ humidified chamber. Non-enzymatic Cellstripper (CelGro, Manassas, VA) was used for cell harvesting. Antibodies used for immunofluorescence microscopy include: anti-α6 integrin (A6NT, (21 (link))), anti-Desmin (Atlas Antibodies HPA018803), anti-E-Cadherin M168 (Abcam ab76055), anti-CK5/14 (KA2), anti-CK18 (Abcam EPR1626), and anti-FOXA1 (Abcam EPR10881). Antibodies used for flow cytometry include: anti-α6 integrin phycoerythrin (PE) conjugated GoH3 (eBioscience, San Diego, CA) and ADG3937 (Sekisui Diagnostics) mouse monoclonal antibody against uPAR. Alexa Fluor 488 (Invitrogen) secondary antibody was used for uPAR detection.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Rubenstein C.S., Gard J.M., Wang M., McGrath J.E., Ingabire N., Hinton J.P., Marr K.D., Simpson S.J., Nagle R.B., Miranti C.K., Warfel N.A., Garcia J.G., Arif-Tiwari H, & Cress A.E. (2019). Gene editing of α6 integrin inhibits muscle invasive networks and increases cell-cell biophysical properties in prostate cancer. Cancer research, 79(18), 4703-4714.

Publication 2019

EPR10881 ADG3937 Alexa Fluor 488

Alexa fluor 488 Antibodies Antibody Cadherin Cell Cell line Cultured medium Desmin Diagnostics Enzymatic Fetal bovine serum Flow cytometry Foxa1 Immunofluorescence microscopy Integrin Monoclonal antibody Mouse Phycoerythrin Upar

Corresponding Organization : University of Arizona

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Cell line: DU145 cell line obtained from American Tissue Type Collection (ATCC)
Cell culture medium: Iscove's modified Dulbecco's medium (IMDM) from Mediatech, supplemented with 10% fetal bovine serum (FBS) from Hyclone Laboratories
Cell culture conditions: Incubated at 37°C in a 5% CO2 humidified chamber
Cell harvesting method: Non-enzymatic Cellstripper (CelGro) used for cell harvesting

controls

No positive or negative controls explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!