The cell populations in BAL were characterized by their specific surface markers [33 (link)] as described in [24 (link)]. Briefly, BAL fluid was centrifuged at 1,000 g for 10 min. The resulting cells were suspended in PBS (105 in 100 μL PBS), transferred to low-binding polypropylene 96-well plates (Corning; Avon, France) and analysed with a MACSQuant® Analyser flow cytometer (Miltenyl Biotec); data for at least 10,000 events were recorded.
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