Isolation of Postnatal Striatal Neurons

Primary neuronal cultures from the striatum of postnatal day 0 (P0) pups were prepared as described previously [24 (link)]. In brief, neurons were dissociated by papain buffer (Sigma), and were then placed in the poly-D-lysine coated slides (BD) or plates in Basal Eagle Medium (Sigma). Arabinosylcytosine (Sigma) was used to inhibit glial cell growth. Tetrodotoxin (TTX, Sigma), Bicuculline (Sigma) and LRRK2 kinase inhibitor MLi-2 (Tocris Bioscience, Bristol, UK) were added directly to the medium of striatal neurons from the stock solutions.

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Chen X., Xie C., Tian W., Sun L., Wang Z., Hawes S., Chang L., Kung J., Ding J., Chen S., Le W, & Cai H. (2020). Parkinson’s disease-related Leucine-rich repeat kinase 2 modulates nuclear morphology and genomic stability in striatal projection neurons during aging. Molecular Neurodegeneration, 15, 12.

Publication 2020

papain buffer Basal Eagle Medium Arabinosylcytosine Tetrodotoxin (TTX, Bicuculline MLi-2

Arabinosylcytosine Bicuculline Buffer Eagle Glial cell Inhibit Kinase Lrrk2 Lysine Neurons Papain Poly Striatal

Corresponding Organization :

Other organizations : National Institutes of Health, National Institute on Aging, Ruijin Hospital, Shanghai Jiao Tong University, First Affiliated Hospital of Dalian Medical University, Dalian Medical University

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Variable analysis

independent variables

Tetrodotoxin (TTX, Sigma)
Bicuculline (Sigma)
LRRK2 kinase inhibitor MLi-2 (Tocris Bioscience, Bristol, UK)

dependent variables

Not explicitly mentioned

control variables

Poly-D-lysine coated slides (BD) or plates
Basal Eagle Medium (Sigma)
Arabinosylcytosine (Sigma) to inhibit glial cell growth

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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