Similar procedures were conducted in our previous work (Lan et al., 2016 (link)). The strains were incubated at 28 °C for 72 h, and the mycelia of each sample were collected, then frozen in liquid nitrogen and ground into powder. Next, 100 mg powder was dissolved in 1 mL radio immuno precipitation assay lysis buffer (RIPA, Beyotime, Shanghai, China), which was used to extract the whole proteins. The concentrations of the protein extracts were measured using a Nanodrop detector and then diluted. We uploaded 40 μg of total protein and separated them by 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis. The proteins were then transferred to a polyvinylidene fluoride membrane (Millipore, USA). We used the specific antibodies anti-actin (1:5000 dilution, Cell Signaling Technology), anti-Histone3 (1:5000 dilution, Abcam), anti-acetyl-histone3 (H3ac, 1:5000 dilution, Abcam), anti-acetyl-H3K56 (H3K56ac, 1:5000 dilution, Active Motif), and anti-acetyl-H3K9 (H3K56ac, 1:5000 dilution, PTM Biolabs) to detect histone modifications.
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