Immunoprecipitation and Immunoblotting Analysis

Immunoprecipitation and imunoblotting were performed as described previously [26 (link)]. The reagents were as following: anti-β-catenin (BD Bioscience, Cat# 610153), anti-γ-catenin (BD Bioscience, Cat# 610253), anti-CBP (Santa Cruz, clone SC-369), anti-p300 (Santa Cruz, clone SC-584), anti-activated- β-catenin (Millipore, clone 8E7), anti-lamin A/C (Santa Cruz, SC-7293), NE-PER Nuclear extraction reagent (Pierce, Cat#78833), Protease inhibitor cocktail (Calbiochem, Cat#539137), Protein A-agarose (Roche, Cat#11134515001), Illustra microspin columns (GE Healthcare, Cat#27-3565-01), ECL Plus (GE Healthcare, Cat#RPN 2132), and Blue ultra autorad film (BioExpress, Cat# F9029-8X10).

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Zhao Y., Wu K., Nguyen C., Smbatyan G., Melendez E., Higuchi Y., Chen Y, & Kahn M. (2017). Small molecule p300/catenin antagonist enhances hematopoietic recovery after radiation. PLoS ONE, 12(5), e0177245.

Publication 2017

anti-β-catenin anti-γ-catenin anti-CBP anti-p300 anti-activated- β-catenin anti-lamin A/C Protein A-agarose Illustra microspin columns ECL Plus

Agarose Catenin Clone Immunoprecipitation Lamin a c P300 Protease inhibitor Protein a β catenin

Corresponding Organization : Osaka University

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Variable analysis

independent variables

Anti-β-catenin antibody (BD Bioscience, Cat# 610153)
Anti-γ-catenin antibody (BD Bioscience, Cat# 610253)
Anti-CBP antibody (Santa Cruz, clone SC-369)
Anti-p300 antibody (Santa Cruz, clone SC-584)
Anti-activated-β-catenin antibody (Millipore, clone 8E7)
Anti-lamin A/C antibody (Santa Cruz, SC-7293)

dependent variables

Not explicitly mentioned

control variables

NE-PER Nuclear extraction reagent (Pierce, Cat#78833)
Protease inhibitor cocktail (Calbiochem, Cat#539137)
Protein A-agarose (Roche, Cat#11134515001)
Illustra microspin columns (GE Healthcare, Cat#27-3565-01)
ECL Plus (GE Healthcare, Cat#RPN 2132)
Blue ultra autorad film (BioExpress, Cat# F9029-8X10)

controls

Positive controls: Not specified
Negative controls: Not specified

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