Measuring Cellular ATP and Mitochondrial Function

The cellular ATP levels were measured using a firefly luciferase-based ATP assay kit (Beyotime) based on a fluorescence technique (Genmed Scientifics Inc.) as previously described (31 (link)). The JC-1 kit (Beyotime) was applied to assess changes in the mitochondrial membrane potential (ΔΨm). The mPTP opening was observed as a rapid dissipation of tetramethylrhodamine ethyl ester (TMRE) fluorescence according to a previous study (28 (link)).

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Hu Y., Wang B., Wang L., Wang Z., Jian Z, & Deng L. (2020). Mammalian STE20-like kinase 1 regulates pancreatic cancer cell survival and migration through Mfn2-mediated mitophagy. Molecular Medicine Reports, 22(1), 398-404.

Publication 2020

firefly luciferase-based ATP assay kit fluorescence technique JC-1 kit

Assay Cellular Firefly luciferase Fluorescence Mitochondrial membrane potential Mptp Tetramethylrhodamine ethyl ester

Corresponding Organization :

Other organizations : Guilin Medical University

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Variable analysis

independent variables

Cellular ATP levels

dependent variables

Mitochondrial membrane potential (ΔΨm)
Mitochondrial permeability transition pore (mPTP) opening

control variables

Unspecified

controls

Positive control: Unspecified
Negative control: Unspecified

Annotations

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