Total RNA, including the miRNA fraction, was extracted from both plasma and supernatants obtained from in vitro studies by using the QIAzol miRNeasy kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions13 (link) (Online Supplementary Appendix). To identify the changes that occurred in the expression levels of miRNAs in plasma from APS patients and HDs, a Human Serum & Plasma miRNA PCR-array (Qiagen) was performed (Online Supplementary Appendix) on an exploratory cohort (Online Supplementary Table S2).
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Extracting and Analyzing miRNA from Plasma
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Other organizations : Instituto Maimónides de Investigación Biomédica de Córdoba, Hospital Universitario Reina Sofía, University of Córdoba, Hospital General Universitario Morales Meseguer, Centro Regional de Hemodonación, Universidad de Murcia, University of Turin, St Thomas' Hospital
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- Extraction of total RNA, including the miRNA fraction, from plasma and supernatants obtained from in vitro studies
- Expression levels of miRNAs in plasma from APS patients and healthy donors (HDs)
- Extraction of total RNA, including the miRNA fraction, was performed using the QIAzol miRNeasy kit (Qiagen, Valencia, CA, USA) following the manufacturer's instructions
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