Substrate 4-nitrophenyl butyrate (pNPB) and salts monosodium phosphate and disodium phosphate, sodium acetate and sodium borate were purchased from Sigma-Aldrich. Zeocine was purchased from Invitrogen. All the media components used (Liu et al. 2009 (link)) were also procured from Sigma-Aldrich. The polymeric substrates polycaprolactone (PCL) (mol wt Mn = 84,000) was a gift from DOW. PCL films were casted by the compression molding, and the crystallinity (30– 35 %) was determined using differential scanning calorimetry. Cellulose diacetate (CA) films were obtained form Grafix Plastics, and the degree of substitution of 2 was confirmed by NMR analysis.
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Enzyme Kinetics with Synthetic Polymers
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Butyrate
Cellulose diacetate
Differential scanning calorimetry
Disodium phosphate
Phosphate
Polycaprolactone
Polymeric
Salts
Sodium acetate
Sodium borate
Corresponding Organization :
Other organizations : Rensselaer Polytechnic Institute, New York University, New York University Abu Dhabi
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Variable analysis
independent variables
- Substrate 4-nitrophenyl butyrate (pNPB)
- Monosodium phosphate
- Disodium phosphate
- Sodium acetate
- Sodium borate
- Zeocine
- Media components from Sigma-Aldrich (as described in Liu et al. 2009)
- Polycaprolactone (PCL) (mol wt Mn = 84,000)
- Cellulose diacetate (CA) films
- Crystallinity (30–35%) of PCL films determined using differential scanning calorimetry
- Degree of substitution of 2 in CA films confirmed by NMR analysis
- Compression molding process for casting PCL films
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