Lipopolysaccharide-free Colominic Acid and Histone Experiments

For cell culture experiments, LPS was removed from colominic acid (Gerbu, Heidelberg, Germany) using C18 cartridges (Thermo Fisher Scientific, Dreieich, Germany), according to manufacturer specifications, as already mentioned in [33 (link)]. Used histones were: histone from calf thymus (Sigma-Aldrich, Steinheim, Germany) and recombinant human H1, H2A, H2B, H3.1, and H4 (New England Biolabs, Frankfurt am Main, Germany). All of the reagents used were of analytical grade.

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Zlatina K., Lütteke T, & Galuska S.P. (2017). Individual Impact of Distinct Polysialic Acid Chain Lengths on the Cytotoxicity of Histone H1, H2A, H2B, H3 and H4. Polymers, 9(12), 720.

Publication 2017

C18 cartridges histone from calf thymus

Cell culture Colominic acid Histone Human Thymus

Corresponding Organization : Research Institute for Farm Animal Biology (FBN)

Other organizations : University of Giessen

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Variable analysis

independent variables

Colominic acid (Gerbu, Heidelberg, Germany) with LPS removed using C18 cartridges (Thermo Fisher Scientific, Dreieich, Germany)
Histones: histone from calf thymus (Sigma-Aldrich, Steinheim, Germany) and recombinant human H1, H2A, H2B, H3.1, and H4 (New England Biolabs, Frankfurt am Main, Germany)

dependent variables

Not explicitly mentioned

control variables

All of the reagents used were of analytical grade

controls

Not specified
Not specified

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